FIG. 4.

Tdcrz1Δ cells exhibit specific phenotypes in response to diverse ionic stresses. (A) Exponentially growing cultures of the T. delbrueckii strains PYCC5321 (wild type [wt]) and MJH211 (Tdcrz1Δ) were adjusted to an OD₆₀₀ of 0.3, diluted (1 to 10⁻⁴), and spotted (3 μl) onto YPD agar medium, YPD adjusted to pH 8.0, or YPD supplemented with MnCl₂, CaCl₂, NaCl, or LiCl at the indicated concentrations. Plates were incubated at 30°C for 2 to 5 days. A representative experiment is shown. (B) Tdcrz1Δ mutant cells were transformed with plasmid pAMS354, which contains the S. cerevisiae CRZ1 gene, and transformants (Tdcrz1Δ CRZ1) were examined for growth on MnCl₂ medium. The wild-type strain PYCC5321 (wt) and the mutant strain MJH211 (Tdcrz1Δ) were used as controls. Cells were grown in liquid YPD containing 0.2 M CaCl₂, diluted as described for panel A, and spotted onto YPD agar medium lacking or containing MnCl₂ at the indicated concentrations. In all cases, a representative experiment is shown.