FIG. 7.

Activity of TdCrz1p is not required for the salt-induced transcriptional activation of the TdENA1 gene. YPD-grown cells of the T. delbrueckii strains PYCC5321 (wild type [wt], ▪) and MJH211 (Tdcrz1Δ, □) were transferred to 1.4 M NaCl-YPD or 0.5 M LiCl-YPD lacking (A) or containing (B) 5 μg/ml FK506. Samples were taken at the indicated times and analyzed by Northern blotting, as described in Materials and Methods. Filters were probed for TdENA1 mRNA. The graphs represent quantification of the mRNA levels of TdENA1 relative to those of the S. cerevisiae ACT1 gene. Results from a representative experiment are shown.