TABLE 2.

Oligonucleotides used in this study

Primer	Sequence (5′ to 3′)	Comment(s)
FR55	ATGACCATGATTACGCCAA	TdENA1 and TdCRZ1 sequencing
FR77	GTAAAACGACGGCCAGT	TdENA1 and TdCRZ1 sequencing
FR94	AATCATCGGCAACCTTAG	TdCRZ1 sequencing
FR103	GGCGACATGCTACGACTTC	TdCRZ1 sequencing
FR136	GTGGTTAAATAGGACATCGC	TdCRZ1 sequencing
FR90	TGCTACTGAAAAGACAAGG	TdENA1 sequencing
FR96	GCTTACAGGCGAGGAATT	TdENA1 sequencing
FR135	TGAGTCCTTGCCTATCGC	TdENA1 sequencing
FR110	CCTCGTTCTGCTTTGACA	TdENA1 sequencing
FR117	CAGGATATCAAGGGTAAGCT	TdENA1 sequencing
FR376	AATGGTTCAGACGTCGC	TdENA1 sequencing
FR377	CCAGCTGATCACTTCGG	TdENA1 sequencing
FR101	CCTAAAGCCCAAACTATAACA	TdCRZ1 sequencing, verify correct targeting of the natMX4 module
FR142	CGAAGTCGACAGCTCAATCA	PCR amplification of TdCRZ1 3′ side
FR141	TGAATTCGGGTAAGAAAAGG	PCR amplification of TdCRZ1 3′ side
FR140	CATTGAGCTCCTTGGAAGG	PCR amplification of TdCRZ1 5′ side
FR139	ATTCGGATCCTAAGTCACTC	PCR amplification of TdCRZ1 5′ side
FR126	TTCAGTGCCGAAGGGACTAC	Verify correct targeting of the natMX4 module
FR76	GTCAAGGAGGGTATTCTGG	Verify correct targeting of the natMX4 module
FR75	AGTTAAGTGCGCAGAAAG	Verify correct targeting of the natMX4 module
FR96	GCTTACAGGCGAGGAATT	TdENA1 probe for Northern blotting
FR121	GCTGCACCAACAGACAAAG	TdENA1 probe for Northern blotting
FR390	GGTATGTTCTAGCGCTTG	ACT1 probe for Northern blotting
FR391	TCTGGGGCTCTGAATCTT	ACT1 probe for Northern blotting