FIG. 2.

Interaction of Grb14 with ZIP in vitro. (A) ZIP isoforms expressed as GST-fusion proteins or GST control were immobilized on glutathione-Sepharose beads and incubated with lysates of CHO-IR-Grb14 cells. Bound proteins were immunodetected with anti-Grb14 polyclonal antibodies. (B) Grb7 family members expressed as immobilized GST-fusion proteins were incubated with lysates of COS cells transiently transfected with V5 tagged ZIPα. Bound proteins were detected by immunodetection with anti-V5 monoclonal antibodies. These blots are representative of 3 different experiments.