FIG. 4.

Mapping of domains involved in the Grb14-ZIP interaction. (A) ZIPα and various ZIP constructs expressed as GST fusion proteins were incubated with lysates of CHO-IR-Grb14 cells. Bound proteins were immunodetected with anti-Grb14 antibodies. (B) Various Grb14 constructs expressed as GST fusion proteins were incubated with lysates of COS cells transiently transfected with V5-ZIPα. Bound proteins were immunodetected using anti-V5 antibodies. Ponceau red staining of the proteins after transfer confirmed that equivalent amounts of GST fusions were loaded. These blots are representative of three different experiments.