FIG. 1.

GPI anchor mutants have altered gross and hyphal morphologies. Panels A to F are photographs of strains that were inoculated on agar growth medium in standard petri dishes. Panels G to L are pictures of the same strains that were inoculated between two cellophane sheets on agar growth medium, and the growing edge of each colony was photographed at a magnification of ×400. All cultures were incubated at room temperature, with the exception of the 34-15 (gpig-1) temperature-sensitive mutant, which was grown at 39°C to induce the mutant phenotype. Colonies of the wild-type (GTH-16) strain (A and G), MSA-7 (gpip-1) mutant (B and H), 34-15 (gpig-1) mutant (C and I), gpip-2 mutant (D and J), gpip-3 mutant (E and K), and gpit-1 mutant (F and L) are shown. The images in panels A to D, H, and J to L were captured at 48 h after inoculation. Panels E and F are shown at 10 days after inoculation. Panel G is shown at 24 h after inoculation. For the micrograph in panel I, the 34-15 (gpig-1) mutant was initially grown at room temperature for 24 h and then shifted to 39°C for an additional 6 h prior to examination. The scale bar in panel L represents a distance of 10 μm.