FIG. 4.

Cell wall absorption of aniline blue dye. Purified cell walls were prepared from the gpip-3 and gpit-1 mutants, the mnt-1 mutant, and the wild-type (GTH-16) parental strain as described in Materials and Methods. Increasing amounts of the mutant and wild-type cell wall preparations were incubated with a solution of 0.002% aniline blue dye, and the amount of aniline blue dye absorbed by each was determined as described in Materials and Methods. Graphed values are the means ± standard deviations of the results from three independent determinations.