FIG. 5.

The gpig-1 mutant lacks a number of “cell wall-associated” proteins at the restrictive temperature. The gpig-1 temperature-sensitive mutant and wild-type strain were grown at the permissive (22°C) and restrictive (39°C) temperatures and harvested, and cell extracts of each were prepared. Aliquots of the SDS-soluble material from each cell extract were separated by SDS-PAGE and analyzed by Western blotting using an “anti-cell wall” antibody. Samples of the gpig-1 mutant at 22°C (lane 1) and 39°C (lane 2) and the wild-type strain at 22°C (lane 3) and 39°C (lane 4) are shown. The molecular masses indicated at the right are in kilodaltons.