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Ssy5p 45-kDa C-terminal fragment can be isolated from fractions eluted with imidazole. Samples from the fractions collected during the 500 mM imidazole elution step (fractions 42 to 52) were analyzed by Coomassie staining of the proteins separated on a 4 to 12% gradient NuPAGE Bis-Tris gel. Ssy5p-C, ca. 45-kDa C-terminal Ssy5p protease fragment; M, BenchMark protein molecular size markers.
