TABLE 1.
Strains used in this study
| Strain | Genotypea | Source or reference |
|---|---|---|
| S. cerevisiae | ||
| M3124 | MATaura3-52 leu2-3 leu2-112 his3-Δ200 prc1-Δ::HIS3 prb1-Δ::LEU2 pep4-d1137 | 35 |
| M5359 | MATaSUC2 mal gal2 CUP1 ura3 gap1Δ AGP1::PAGP1-lacZ | 28 |
| M5360 | MATaSUC2 mal gal2 CUP1 ura3 gap1Δ ptr3Δ AGP1::PAGP1-lacZ | 28 |
| M5361 | MATaSUC2 mal gal2 CUP1 ura3 gap1Δ ssy5Δ AGP1::PAGP1-lacZ | 28 |
| M5380 | MATaSUC2 mal gal2 CUP1 ura3 gap1Δ ssy1Δ AGP1::PAGP1-lacZ | 28 |
| M5444 | MATaSUC2 mal gal2 CUP1 ura3 gap1Δ ssy5Δ STP1::ZZ-kanMX | 28 |
| E. coli | ||
| BL21(DE3) Codon Plus RIL | B F−ompT hsdS(rB− mB−) dcm+ Tetrgal λ(DE3) endA Hte(argU ileY leuW Camr) | Stratagene |
| XL10 | Tetr Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F′ proAB lacIqZΔM15] Tn10(Tetr) Amy Camr | Stratagene |
a
Tet, tetracycline; Cam, chloramphenicol.