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. 2006 Mar;5(3):601–608. doi: 10.1128/EC.5.3.601-608.2006

TABLE 2.

Primers used for introduction of site-specific mutations in SSY5

Primer DNA sequence (5′→3′)
E131K TCT TGA GTC CTG TTA AGA AGG AGG AAT CTC AGG ATA C
Anti E131K GTA TCC TGA GAT TCC TCC TTC TTA ACA GGA CTC AAG A
F575V CAT TCC CAG ATC CAA CAT TAA GAG TTC AAA ATT TAC ATG TGA AAC GAA AAA TT
Anti F575V AAT TTT TCG TTT CAC ATG TAA ATT TTG AAC TCT TAA TGT TGG ATC TGG GAA TG
Q576P CCC AGA TCC AAC ATT AAG ATT TCC AAA TTT ACA TGT GAA ACG AAA AAT T
Anti Q576P AAT TTT TCG TTT CAC ATG TAA ATT TGG AAA TCT TAA TGT TGG ATC TGG G
K581N CAA CAT TAA GAT TTC AAA ATT TAC ATG TGA ATC GAA AAA TTT TTA AAA TGA AGC CTG G
Anti K581N CCA GGC TTC ATT TTA AAA ATT TTT CGA TTC ACA TGT AAA TTT TGA AAT CTT AAT GTT G
P632H GCG AGT TTG TCG TTG CAT CTC CAA CTC ATT TAT TTG CTA GTG CGG GGG ATT CAG
Anti P632H GCC TGA ATC CCC CGC ACT AGC AAA TAA ATG AGT TGG AGA TGC AAC GAC AAA CTC