Skip to main content
. Author manuscript; available in PMC: 2006 Sep 1.
Published in final edited form as: Hepatology. 2005 Sep;42(3):568–577. doi: 10.1002/hep.20821

Fig. 5. Effects of dietary fatty acids and ethanol on adiponectin production in 3T3-L1 adipocytes.

Fig. 5

(A) The differentiated 3T3-L1 adipocytes were incubated for 16 h in serum-free DMEM and then treated with various fatty acid:BSA complexes in the absence or presence of ethanol as indicated. Analysis of adiponectin concentrations in the culture media was performed using an ELISA-based assay. (B) Hela cells were transfected with a mouse adiponectin promoter-luciferase reporter and expression plasmids for PPARγ and RXRα. Following transfection, cells were treated with various fatty acid:BSA complexes in the absence or presence of ethanol. Rosiglitazone (10 μM) was used as a positive control. Normalized luciferase activities are shown as mean ± SE from at least three experiments performed in duplicate. *p < 0.05; **p < 0.01 in comparison with controls.