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. 2002 Oct;22(20):6993–7003. doi: 10.1128/MCB.22.20.6993-7003.2002

FIG. 3.

FIG. 3.

Overexpression of TEF1 suppresses CPR1 dependence and cycloheximide sensitivity of the zpr1-1 strain. (A) Strain HC17-5C zpr1-1 cpr1Δ cells with pCH1122-CPR1 (CPR1/ADE3/URA3) were transformed with empty vector, pRS415-CPR1 (CEN), or pRS425-TEF1 (2μm) and then serially diluted 1:5, plated onto 5-FOA plates, and grown for 5 days at 30°C. (B) ZPR1 (W303-1A) and zpr1-1 (HC21-11D) cells containing empty vector, pRS414-ZPR1 (CEN), or pRS425-TEF1 (2μm) were plated onto SC containing 40 ng of cycloheximide per ml, 1 ng of rapamycin per ml, or 4 mg of paromomycin per ml in fivefold serial dilutions.