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. 2002 Oct;22(20):6993–7003. doi: 10.1128/MCB.22.20.6993-7003.2002

FIG. 4.

FIG. 4.

The absence of Cpr1p and PPIase activity results in nuclear accumulation of Zpr1p. (A) CPR1 ZPR1-GFP (HC22-1B) cells containing pRS414 and cpr1Δ ZPR1-GFP (HC24-8A) cells containing either pRS414 or pRS414-CPR1 were grown to log phase in SC lacking tryptophan and examined by fluorescence microscopy. (B) (Left) Wild-type (HC22-1B) and cpr1Δ (HC24-8A) cells expressing Zpr1p-GFP were grown to log phase in SC plus glucose, starved in glucose-free SC for 18 h, and then refed glucose. (Right) Wild-type (HC22-1B) and cpr1Δ (HC24-8A) cells expressing Zpr1p-GFP were grown to log phase, poisoned with sodium azide-deoxyglucose for 45 min, washed, and resuspended in SC plus glucose. The percentages of cells displaying predominantly nuclear fluorescence at the indicated times are shown. (C) CPR1 ZPR1-GFP (HC22-1B) cells containing empty vector (pRS414) and cpr1Δ ZPR1-GFP (HC24-8A) cells containing either empty vector (pRS414) or pRS414 bearing the indicated PPIases were grown to log phase in SC lacking tryptophan and examined by fluorescence microscopy. The percentages of cells displaying predominantly nuclear fluorescence are shown.