TABLE 2.
Plasmids used in this study
| Plasmid(s) | Insert or marker | Copy no. | Source or reference |
|---|---|---|---|
| pRS plasmids | Stratagene | ||
| pCH1122 | ADE3/URA3 | CEN | 22 |
| pCH1122-CPR1 | CPR1/ADE3/URA3 | CEN | This study |
| pBM272 | GALp/URA3 | CEN | 20 |
| pBM272-CPR1 | GALp-CPR1/URA3 | CEN | This study |
| pRS414-PT | CPR1p-CPR1t/TRP1 | CEN | This study |
| pRS414-CPR1 | CPR1/TRP1 | CEN | This study |
| pRS414-hCyPA | hCyPA/TRP1 | CEN | This study |
| pRS414-H54Q | hCyPA-H54Q/TRP1 | CEN | This study |
| pRS414-H126Q | hCyPA-H126Q/TRP1 | CEN | This study |
| pRS414-ZPR1 | ZPR1/TRP1 | CEN | This study |
| pRS415-PT | CPR1p-CPR1t/LEU2 | CEN | This study |
| pRS415-CPR1 | CPR1/LEU2 | CEN | This study |
| pRS415-hCyPA | hCyPA/LEU2 | CEN | This study |
| pRS415-H54Q | hCyPA-H54Q/LEU2 | CEN | This study |
| pRS415-F55A | hCyPA-R55A/LEU2 | CEN | This study |
| pRS415-F60A | hCyPA-F60A/LEU2 | CEN | This study |
| pRS415-F113A | hCyPA-F113A/LEU2 | CEN | This study |
| pRS415-W121A | hCyPA-W121A/LEU2 | CEN | This study |
| pRS415-H126Q | hCyPA-H126Q/LEU2 | CEN | This study |
| pRS425-TEF1 | TEF1/LEU2 | 2μm | This study |
| YEplac181-CPR6 | CPR6/LEU2 | 2μm | This study |
| YEplac181-FPR1 | FPR1/LEU2 | 2μm | This study |
| pFPR4 | FPR4/LEU2 | 2μm | J. Heitman |
| pESS1 | ESS1/HIS3 | 2μm | S. Hanes |