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. 2002 Oct;22(20):7093–7104. doi: 10.1128/MCB.22.20.7093-7104.2002

FIG. 2.

FIG. 2.

SLBP must bind mRNA to stimulate translation. (A) Lysates containing hSLBP or two mutant hSLBPs that do not bind the stem-loop were incubated with the standard polyadenylated CAT mRNA and the Luc-TL or Luc-SL mRNA and assayed for luciferase activity. hSLBPRR/KK has arginines 10 and 11 in the RBD changed to lysines, and hSLBPYY/SS has tyrosines 24 and 27, also in the RBD, changed to serines (12). (B) Lysates containing xSLBP1 were incubated with the standard polyadenylated CAT mRNA and the Luc-TL or Luc-SL mRNA in the presence of either excess 30-nt RNA containing the histone stem-loop (SL) or with the reverse stem-loop (RS) and then assayed for luciferase activity.