FIG. 5.

Assay of the effects of xSLBP chimeras on translation in vitro. Lysates containing the xSLBP chimeras shown in Fig. 4 were incubated with the standard polyadenylated CAT mRNA and the Luc-TL or Luc-SL mRNA and assayed for luciferase activity. The reaction mixtures were analyzed by gel electrophoresis, and the proteins were detected by autoradiography (top). Luciferase activity was assayed by luminometry, and the fold activation was expressed as the ratio of Luc-SL activity to Luc-TL activity (bottom). The bar graph shows the averages of two experiments with two different batches of RRL, and the error bars represent the standard deviations.