FIG. 6.

Effect of xSLBP1 deletion mutant forms on translation activation in vitro. (A) Deletions in the amino- and carboxy-terminal regions of xSLBP1. (B) Lysates containing the xSLBP1 deletion mutant forms shown in panel A were incubated with the standard polyadenylated CAT mRNA and the Luc-TL or Luc-SL mRNA. Protein synthesis (top) and luciferase activity (bottom) were assayed as described in Fig. 1C and D, respectively. The bar graph shows the averages of two experiments with two different batches of RRL, and the error bars represent the standard deviations.