FIG. 9.

Translation activation by MS2-hSLBP fusion protein in vitro. Lysates containing MS2 (lanes 1 to 3), MS2-hSLBP fusion protein (lanes 4 to 6), or hSLBP (lanes 7 to 9) were incubated with the standard polyadenylated CAT mRNA and the Luc-TL, Luc-SL, or Luc-MS2 mRNA. Protein synthesis (top) and luciferase activity (bottom) were assayed as described in Fig. 3C and D, respectively. The bar graph shows the averages of three experiments with two different batches of RRL, and the error bars represent the standard deviations.