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. 2002 Oct;22(20):7313–7324. doi: 10.1128/MCB.22.20.7313-7324.2002

FIG. 3.

FIG. 3.

Effect of homozygous Af9 mutation on embryonic development. Heterozygous Af9LZ or Af9NO mice were mated, and embryos were removed at E12.5 (A) or E14.5 (B). All embryos were prefixed in 4% PFA and stained with X-Gal solution overnight. (A) Comparison of β-galactosidase staining patterns of Af9LZ and Af9NO heterozygous embryos at E12.5. Similar staining patterns were observed with both lines. (B) Comparison of β-galactosidase staining patterns of heterozygous and homozygous Af9NO embryos. An Af9NO+/− embryo (top) is compared with an Af9NO−/− embryo (bottom; E14.5). A dorsal view of each embryo is shown on the left, with an enlargement of the cervical region on the right. Staining along the axial skeleton extended to a more anterior limit in the homozygous Af9 mutants. In addition, the structures exhibiting Af9 expression appeared to spread over a broader lateral area in this region.