FIG. 6.

Deletion of hri1⁺ and hri2⁺ does not significantly alter growth in rich or minimal medium. S. pombe strains SP223 (▪, hri1⁺ hri2⁺), WY457 (♦, Δhri1 hri2⁺), WY458 (▴, hri1⁺ Δhri2), and WY459 (•, Δhri1 Δhri2) were grown in YES (A) or EMM (B). Aliquots were removed from each culture at the listed times and monitored for absorbance at 600 nm. (C) S. pombe SP223-derived strains with the indicated genotypes were grown to mid-exponential phase in YES medium at 30°C, and equal amounts of lysate prepared from each culture were analyzed by immunoblot using antibody that specifically recognizes eIF2α phosphorylated at Ser-51 (eIF2α−P) or antiserum that detects total eIF2α protein.