FIG. 8.

Loss of hri1⁺ and hri2⁺ impairs phosphorylation of eIF2α in response to stress conditions. The S. pombe SP223-derived strains with the indicated genotypes were grown to mid-exponential phase in YES medium supplemented with all 20 amino acids at 30°C and subjected to the presence or absence of heat shock (A), sodium arsenite (B), or sodium arsenate (C), as described in the Materials and Methods. Equal amounts of lysate prepared from each culture were analyzed by immunoblotting using antibody that specifically recognizes eIF2α phosphorylated at Ser-51 (eIF2α−P) or antiserum that detects total eIF2α protein (bottom panel).