FIG. 7.

Activation of endosome-associated EGFR and the effects on cell survival. BT20 and MDCK cells were incubated in serum-free medium to induce apoptosis. The cells were then treated AG-1478 and EGF (20 ng/ml) with or without monensin followed by washing and incubation as described above. To determine the effects of wortmannin, the cells were pretreated with wortmannin (100 nM). The apoptosis was determined by TUNEL assay.