TABLE 1.
Analysis by subcellular fractionation of the selective activation of endosome-associated EGFR
| Cell type and marker | Yield (%) of indicated fractiona for:
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Control cells
|
Cells treated with AG1478 + EGF + monensin
|
|||||||||||
| Without EGF
|
With EGF
|
Without wash
|
With wash
|
|||||||||
| CY | PM | EN | CY | PM | EN | CY | PM | EN | CY | PM | EN | |
| BT20 | ||||||||||||
| EGFR | 0 | 78 | 22 | 0 | 28 | 72 | 0 | 9 | 91 | 0 | 10 | 90 |
| p-EGFR | 0 | 0 | 0 | 0 | 27 | 73 | 0 | 0 | 0 | 0 | 2 | 98 |
| EEA1 | 29 | 8 | 63 | 3 | 17 | 80 | 2 | 35 | 63 | 4 | 2 | 94 |
| MDCK | ||||||||||||
| EGFR | 0 | 94 | 5 | 5 | 15 | 80 | 0 | 4 | 95 | 2 | 2 | 96 |
| p-EGFR | 0 | 0 | 0 | 0 | 9 | 91 | 0 | 0 | 0 | 0 | 3 | 97 |
| EEA1 | 12 | 7 | 81 | 5 | 14 | 81 | 3 | 19 | 78 | 3 | 6 | 91 |
a
Data are the quantification of the results from Fig. 2A normalized against the total yield of the three fractions. Each value is the percentage of the total of each set of three fractions following normalization.