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. 2000 Mar;12(3):319–342. doi: 10.1105/tpc.12.3.319

Table 2.

Proteins Involved in Nonphotosynthetic Functions, Identified from the Two-Dimensional Electrophoresis Gels of the Lumenal and Peripheral Fractions (pI 4.0 to 7.0 and pI 7.0 to 11.0) Shown in Figures 2A to 2Da

MALDI-
TOF
Edmane or ESI-MS/MSf
Localization and Cleavage
Site Prediction
Spot No. Masses (kD) pI Identityb(Theorical Mass in kD) Accession
Numberc 		Cover %
50 ppmd 		Sequence % of
Identityg 		N Terminus of
the Proteinh 		PSORTi ChloroPj SignalPk
123 37.3 5.6 Hcf136b(44.1) O82660 24 EETLSE-ERVYLe 67 79-DE ER: 0.60
Tk: 0.49		 60–61 ARA-DE
78–79
127 46.0 5.3 DegPb (46.2) 2565436 15 104-FV Tk: 0.91
Lumen: 0.80		 42–43 AVE-SA
99–100
VES-AS
100–101
119 29.9 4.9 RNA binding
  protein (32.0)		 PSY14557 AAQEGETLTVEETVe 86 61-AA Mito: 0.61
Stroma: 0.50		 60–61 LFA-AQ
61–62
124 39.1 4.6 Plastoglobule ass.
  prot. PG1 (38.4)		 4105180 25 48-AG Stroma: 0.52 46–47 ISA-AG
47–48
113 24.2 5.8 Cpn21 (26.9) O65282 ATVVAPKYTAIKe 83 52-AS Stroma: 0.89
Lumen: 0.58		 50–51
(25–26)		 AQS-KP
93–94
VKA-AS
51–52
130 57.5 5.3 Cpn60α (14.4) P08926 26 Stroma: 0.92
Lumen: 0.68		 45–46
(23–24)		 AAA-KD
49–50
131, 132 74.5 5.3 Hsp70 (41.3) 399942 27–35 68-KV Stroma: 0.94
Lumen: 0.74		 66–67
(46–47)		 AVA-AM
83–84
2, 3, 4, 6 9.3–15.3 4.4–6.9 Histone H4-likeb (27.6) 1806283 39–40 I/LSGI/LI/LYEETRf 100 Nucleus: 0.95 None None
40 41.2 5.6 Brittle-1b (16.2) 231654 16 76-DN Mito: 0.83
Stroma: 0.52		 44–45 None
42 49.5 5.8 Stearoyl-ACP
  desat.b (15.5)		 2290402 17 Mito: 0.71 33–34 AMA-ST
60–61
39 37.3 5.7 ACC oxidaseb (39.2) 4090533 19 Cyto: 0.45 None None
112 22.9 6.0 Ferritinb (15.5) AI443623 ATKGSSDNRVLTGVe 64 60-AT Cyto: 0.65 59–60 None
23–28,
  205, 206
126		 27.1–28.5
46.0		 5.3–6.3 Putative ascorbate
  perox/partial cDNAb

Putative ascorbate
  perox/partial cDNAb 		AI490846 XDLIERRQRX(Y/E)Fe 77–93
5.8 AW156024
AW185405
AW185405
AI490846 		TDYEVDI/LI/LTTFTKf243.12-FSAVGI/LGPRfI/LNYEAYTYPRfADLIERRQRSEFQe 92
78
80
93		 89-AD ER: 0.55 45–46 ANA-AD
88–89
110 21.3 6.0 FKBP isomerase
  isolog/partial cDNAb 		AU070407
AW092542 		AGLPTEEKPPLLe 64 117-AG Tk: 0.88
Lumen: 0.70		 57–58 ALA-AG
117–118
a

The numbering corresponds to the two-dimensional electrophoresis gel images in Figures 2A to 2D. Proteins were identified by MALDI-TOF, ESI-MS/MS, or N-terminal Edman degradation, as indicated. In case of identification by MALDI-TOF alone, the matching tryptic peptides covered >15% at 50 ppm. Predicted cleavage sites of the chloroplast transit peptide (ChloroP) and the lumenal transit peptide (SignalP), as well as the prediction for the cellular localization (PSORT), are indicated.

b

The species is not pea; instead, Hcf136 and DegP were identified from Arabidopsis, Histone H4-like from Sesbania rostrata, Brittle-1 from maize, Stearoyl-ACP desaturase from Helianthus annuus, ACC oxidase from Carica papaya, ferritin from Glycine max, the putative ascorbate peroxidase (partial cDNAs) from Lycopersicum esculentum (AI490846), Physcomytrella patens (AW156024), and G. max (AW185405), and finally the FKBP-type peptidyl-prolyl cis-trans isomerase was identified from Oryza sativa (AU070407) and from L. esculentum (AW092542).

c

Accession number in NCBI, SWISS-Prot, or PIR databases.

d

Percentage of coverage at 50 ppm for the MALDI-TOF peptides.

e

N-terminal Edman sequence tag.

f

Sequence tag from ESI-MS/MS.

g

Percentage of identity between the amino acids present in the Edman tag or the MS/MS tag and the sequences found in the databases.

h

N terminus of the mature protein given in the databases or by homology, e.g., (39).

i

Localization prediction by PSORT: ER, endoplasmic reticulum; Tk, thylakoid; Mito, mitochondria; Cyto, cytosol. Numbers following colons indicate probability.

j

Cleavage site prediction of the lumenal transit peptide by ChloroP.

k

Cleavage site prediction of the lumenal transit peptide by SignalP.