Figure 1.
Interaction between GNOM Subunits and Mapping of the Interaction Domain in Yeast Two-Hybrid Assays.
(A) GNOM fragments fused to an activation domain (AD–GNOM) tested for interaction with two LexA–GNOM fusions are represented by bars with amino acid positions indicated. Amino acids 1 to 246 encoded by the first exon and the Sec7 domain (Sec7D) are shaded. Vector, negative control.
(B) Interaction of AD–GNOM fragments with nearly full-length GNOM protein fused to a DNA binding domain (LexA–GNOM18–1451).
(C) Interaction of AD–GNOM fragments with N-terminal 246 amino acids of GNOM protein fused to a DNA binding domain (LexA–GNOM1–246).
Activation of leucine growth reporter (-Leu growth) is indicated by ++ or −; LacZ reporter activity is displayed as relative β-galactosidase units determined by liquid culture assay (Ausubel et al., 1995). Error bars represent standard deviations from three to five independent transformants.