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Interaction between GNOM Subunits in Vitro.
In vitro binding assays of the GST–GNOM1–246 fusion are shown.
(A) Arabidopsis protein extracts (protein gel blot; 165-kD band detected by anti-GNOM Sec7 antiserum).
(B) In vitro–translated 35S-methionine–labeled GNOM1–246 (autoradiograph of 15% SDS–polyacrylamide gel).
The controls were glutathione–Sepharose beads (beads) and GST-coupled beads (GST).
