Figure 4.

Specificity and Domain Mapping of Cyp5–GNOM Interaction in the Yeast Two-Hybrid System.

(A) Interaction of the activation domain (AD)–Cyp5 fusion with different LexA–GNOM fragments (amino acid positions indicated). Activation of -Leu growth is indicated by + or −; β-galactosidase activity is given as arbitrary units. Error bars represent standard deviations from five independent transformants. LexA-bicoid (Bicoid) is the negative control.

(B) LexA–Cyp5 interaction with AD–GNOM subfragments (amino acid positions indicated). -Leu growth and β-galactosidase assay are as given in (A).

(C) Specificity of GNOM–Cyp5 interaction compared with other Arabidopsis cyclophilins. LexA–GNOM fusion tested against AD fusions of full-length Cyp5, ROC1, ROC4, and AD vector pJG4.5 (Vector). β-Galactosidase activity and growth on -Leu medium of two transformants streaked on plates and grown for 3 days are shown.