Figure 6.

Alignment of Arabidopsis Cyp5 with Related Cyp Sequences.

Alignment of seven putative cytosolic eukaryotic Cyp proteins as identified and generated by BLAST P and BEAUTY search with multiple sequence alignment (Altschul et al., 1997). Dots indicate amino acid identity; dashes represent gaps. The Cyp5 sequence contains the predicted N-terminal endoplasmic reticulum (ER) transport signal (Saito et al., 1999) and PPIase consensus (PROSITE, underlined). Peptide antigen for rabbit immunization and two nonconserved amino acids (asterisks) involved in cyclosporin A binding of human CypA are underlined (Theriault et al., 1993). An ecotype polymorphism I30V between the Landsberg erecta and Columbia sequences is indicated (top line). The more divergent Arabidopsis ROC4 lacks a seven–amino acid insertion present in the other Cyp proteins.