Figure 3.

Reconstitution of Cortical Microtubule Arrays on Drug-Free Murashige and Skoog Medium after Their Depolymerization by 10 μM Oryzalin.

(A) Cortical microtubules in mature trichomes that had depolymerized during the 30-min oryzalin treatment reappeared after 6 to 12 hr with no discernable change in microtubule distribution or trichome morphology. Note the reappearance of the bright knots (arrowheads).

(B) Trichomes that had been exposed to oryzalin at an early stage of their development also reconstituted their cortical microtubule arrays, but their morphology changed to that of a generally bloated cell.

(C) A trichome in which the cortical microtubules were reconstituted after the drug treatment but were unable to reorganize their normal orientation and also displayed a general shape change.