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. 2000 Apr;12(4):569–582. doi: 10.1105/tpc.12.4.569

Figure 3.

Figure 3.

Figure 3.

Coinfection of Arabidopsis by TEV-GUS and CMV.

Coinfection of wild-type Col-0 and rtm1 and rtm2-1 mutant plants was performed with TEV-GUS (T) and CMV (C). Mutant plants containing rtm1-1, rtm1-2, and rtm1-3 alleles were tested.

(A) Quantitative GUS activity assays of inflorescence tissue from singly infected or coinfected plants sampled at 24 DPI. Each bar represents the mean (±sd) from three plants.

(B) RNA gel blot analysis of CMV RNA3, RNA4, and rRNA in inflorescence tissue from noninfected Col-0 (lane 1), TEV-GUS–infected (lanes 2 to 6), CMV-infected (lanes 7 to 11), and TEV-GUS/CMV–coinfected (lanes 12 to 16) wild-type Col-0 or mutant plants. Equal amounts of inflorescence tissue were pooled from each of three replicate plants before RNA extraction and blotting to nitrocellulose. The CMV RNA3, RNA4, and rRNA probes were labeled with phosphorus-32, and hybridization was detected by exposure to x-ray film.