FIG. 1.

Analysis of Grit amino acid sequence. (A) Schematic representation of human Grit (KIAA0712). The deposited KIAA0712 amino acid sequence (GenBank AB018255) was inferred to be truncated in the middle of the RhoGAP domain; we thus determined the corresponding nucleotide sequences of Grit cDNA clones obtained by 5′ rapid amplification of cDNA ends and added N-terminally 151 residues of Grit. RhoGAP domain, filled box; TrkA/adapter-binding CT region, hatched box. The amino acid positions of the domain boundaries are shown at top. Potential SH3 binding motifs I to V, predicted by the Scansite program, are shown by bars. (B) Phylogenetic analysis of the GAP domains of Grit and RhoGAP family proteins by use of the GENETYX program. The percent identities to human Grit are shown at the right. H.s., Homo sapiens; D.m., D. melanogaster; C.e., C. elegans. (C) Alignment of the GAP domain of Grit with that of other RhoGAP family members. Grit, residues 37 to 190; CdGAP, residues 35 to 188; p190, residues 1263 to 1409; N-chimerin, residues 282 to 434. Identical residues are indicated by asterisks. The critical glycine and arginine for GAP activity are in boldface type.