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The TI domain does not inhibit nuclear localization. Immunofluorescence was used to examine the cellular localization of TAp63α, as described in Materials and Methods. myc-tagged TAp63α was stained with the c-Myc-specific antibody 9E10 (left). The DNA was visualized with the fluorochrome Hoechst 33278 (right). The nuclear staining of TAp63α reveals that the TI domain does not inhibit nuclear localization of the p63 transcription factor.
