FIG. 4.

T₃ treatment of premetamorphic tadpoles increases xN-CoR mRNA levels in the intestine (A) and tail (B) but not in the hindlimb (C). NF55 tadpoles were treated with 5 nM T₃ for the indicated number of days. Total RNA was isolated and subjected to Northern blot analysis (10 μg/lane for tail and intestinal RNA) or RT-PCR analysis (for hindlimb RNA, due to the limited amount of RNA isolated from the small organ) for xN-CoR RNA levels. The signals were quantified with a PhosphorImager and plotted on the right after normalization against the control rpl8 signals. All the experiments were done at least twice with similar results.