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The expression of xSMRT is similar to that of xN-CoR during development. Total RNA was isolated from intestine, tail, and hindlimb from tadpoles at the indicated stages. RT-PCR analysis was carried out to determine the mRNA levels of xSMRT and the internal control gene rpl8. Note that similar to xN-CoR (Fig. 3), xSMRT was upregulated during intestinal remodeling (stage NF60) and tail resorption (stage NF64). The results are from one of two independent experiments with similar results.
