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. 2002 Dec;22(24):8478–8490. doi: 10.1128/MCB.22.24.8478-8490.2002

FIG. 7.

FIG. 7.

Detection of SRE-1 binding proteins in adult mouse spermatogenic cells and tissues. (A) Southwestern analysis of nuclear extracts (5 μg) from enriched spermatogenic cells from adult mouse testis (Gc), brain (B), and liver (Li). Wild-type (SRE-1) and mutant (mSRE-1) probes were used. (B) EMSA of SREBPs in mouse and rat spermatogenic cells. Nuclear extracts (1.5 μg) were examined for spermatogenic cells from adult mouse testis (lanes 2 to 4) and rat testis (lanes 5 to 7) by using the SRE-1 probe. Lanes: 1, probe only; 2 and 5, no competitor; 3 and 6, 10-fold excess of unlabeled SRE-1 wild-type competitor; 4 and 7, 10-fold excess of mutated SRE-1 competitor. The specific SREBP-SRE-1 complex is shown by an asterisk. The residual binding seen with the wild-type SRE-1 competitor was specifically eliminated at larger amounts of competitor (data not shown).