FIG. 8.

Developmental expression of SREBP2gc in male germ cells. (A) Northern analysis of purified mouse spermatogenic cells with rat SREBP2gc cDNA as a probe. Fifteen micrograms (each) of total RNA from spermatogonia type A (A), spermatogonia type B (B), prepubertal pachytene spermatocytes (PP), pachytene spermatocytes (PS), round spermatids (RS), and cytoplasts (Cy) was examined. Relative RNA loading is shown below by the ethidium bromide staining of the blot prior to hybridization. (B) Northern analysis of purified mouse spermatogenic cells with SREBP1c cDNA as a probe. Fractions are as in panel A, with the addition of preleptotene spermatocytes (PL), and the ethidium bromide-stained blot is shown below. (C) Western analysis of SREBP2gc protein in the developing mouse testis. Five micrograms (each) of testis nuclear extracts was examined from the indicated postnatal day mice and adults (Ad). (D) Western analysis of SREBP2gc protein in purified mouse spermatogenic cells. Five micrograms (each) of nuclear extracts from spermatogonia type A (A), spermatogonia type B (B), pachytene spermatocytes (PS), round spermatids (RS), enriched spermatogenic cells from adult mouse testis (Gc), and mouse sperm (SP) was examined.