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Flow-cytometric analysis. Total thymocytes, DN thymocytes, splenocytes, and lymph node cells derived from 4-week-old Lck-Cre (+) STAM1flox/+ STAM2+/− (n = 8) (left) and Lck-Cre (+) STAM1flox/flox STAM2−/− (right) (n = 8) mice were stained with Abs for CD4 and CD8, TCRβ, CD25 and CD44, or CD3 and B220. Numbers indicate the average percentages of the gated cellular subpopulations within the lymphocyte population.
