FIG. 4.

Proliferative responses of thymocytes. Total thymocytes (2 × 10⁵ per well) (A) and CD4 SP thymocytes (5 × 10⁴ per well) (B) were stimulated with indicated ligands: plate-coated anti-CD3 MAb (10 mg/ml), plate-coated anti-CD28 MAb (1 mg/ml), murine IL-2 (10 nM), murine IL-7 (10 ng/ml), phorbol myristate acetate (10 ng/ml), and ionomycin (1 mg/ml). They were cultured for 42 h and were then pulsed with [³H]thymidine and harvested after 6 h.