FIG. 5.

In vitro cell survival of thymocytes and Bcl-2 expression in thymocytes. (A) In vitro cell survival experiment using DP thymocytes and CD4 SP thymocytes. After the indicated culture time, cells were stained with annexin V. Viable cells were defined as annexin-V-negative cells in each fraction of thymocytes. Error bars, standard deviations. (B) Bcl-2 expression levels in thymocyte fractions. Each fraction of thymocytes was stained with either isotopic control MAb (dotted line) or anti-Bcl-2 mouse MAb (solid line). The results, analyzed by gating CD4⁻/CD8⁻ (DN) cells, CD4⁺/CD8⁺(DP), CD4⁺ (CD4 SP), or CD8⁺ (CD8 SP) cells, are shown. Representative results of two independent experiments are presented.