FIG. 7.

Cytokine-mediated signaling in thymocytes. (A) CD4 SP thymocytes were incubated with murine IL-7 for the indicated times and analyzed for phosphorylation of STAT5, ERK, and Akt by immunoblotting. (B) Total thymocytes were incubated with plate-coated anti-CD3 MAb alone (10 mg/ml) or plate-coated anti-CD3 MAb (10 mg/ml) plus murine IL-2 (10 nM) or murine IL-7 (10 ng/ml) for the indicated times. Total RNA prepared from the cells was first hybridized with the c-myc probe and then rehybridized with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probe.