FIG. 4.

Rtg2 is a unique component of SLIK. (A) Rtg2 associates with the Spt7 protein in vivo. Extracts prepared from strains expressing TAP-tagged Rtg2 or Spt8 or untagged control proteins were incubated with calmodulin resin. Inputs and protein eluted from the resin were subjected to Western blotting with antibodies directed against protein A or Spt7. (B) Size-exclusion chromatography of the Rtg2-TAP and Spt8-TAP complexes isolated from the calmodulin resin. Western blotting of Superose 6 fractions was performed with antibodies against protein A or Ada3. (C) Western blots of Rtg2-TAP and Spt8-TAP fractions eluted from Mini Q columns. Fractions from the Superose 6 column were bound to Mini Q columns, and fractions eluted were assayed by Western blotting with protein A antibodies. The relative positions of the SAGA and SLIK complexes are indicated.