FIG. 5.

Effect of deletion of RTG2 on the SLIK and SAGA complexes. (A) HAT assay and Western blot analysis of SLIK and SAGA complexes prepared from wild-type (SFY526) or rtg2Δ strains. Mono Q fractions of partially purified SLIK and SAGA were subjected to nucleosomal HAT assay and Western blotting with Ada2 antiserum. (B) SLIK fractions 27 to 31 eluted from the Mono Q column in panel A were separated on a Superose 6 column. Shown is a fluorogram of a nucleosomal HAT assay performed with Superose 6 fractions from wild-type and rtg2Δ strains. (C) Northern blot analysis of CIT2 expression from wild-type (WT, BY4742), spt7Δ, rtg2Δ, and spt8Δ isogenic strains grown in SC medium containing 2% dextrose (D) or 2% acetate (A) as a carbon source. ACT1 (encoding actin) is shown as a loading control. The relative fold increase in CIT2 RNA levels normalized to control RNA for each strain is shown, as estimated by densitometry.