FIG. 2.

Flow cytometric analysis of B- and T-cell development in the primary and secondary lymphoid organs. Total cells isolated from 3- to 4-month-old mice were stained with the indicated antibodies and analyzed by FACS. The numbers in each region indicate the percentage of the total cells plotted. The data shown are representative of those from a minimum of six pairs of mice examined. (A) Early and late stages (left and right, respectively) of B-cell maturation in the bone marrow. Left panel, B220 versus CD43 profiles of IgM⁻-gated cells; right panels, B220 versus IgM profiles of CD43⁻-gated cells (fractions D, E, and F). (B) Analysis of splenic B and T cells, showing profile of B220 versus Thy1.2 gated lymphocytes. (C) Analysis of peritoneal B1a cells, displayed as the profile of IgM⁺ CD5⁺ cells within the lymphocyte gate. (D) Maturation stages of splenic B cells. Left panels, B220 versus IgM profiles of gated lymphocytes; right panels, IgD versus IgM profiles of gated lymphocytes. (E) Analysis of lymph node B cells, showing B220 IgM profiles of gated lymphocytes. (F) Maturation of thymocytes is unimpaired (CD4 versus CD8 profile of gated thymocytes).