FIG. 6.

Impaired in vitro proliferative response of p110δ-deficient lymphocytes. (A). Purified splenic B cells from 3- to 4-month-old wild-type mice (WT), p110δ^−/− mice, and PLCγ2^−/− mice were incubated in medium alone or in medium containing the indicated concentrations of various stimuli. Proliferative responses are expressed as mean counts of triplicates from a single [³H]thymidine incorporation assay; error bars indicate standard deviations. Results are representative of those from four to six experiments. Ion, ionomycin. (B) Splenic T cells were incubated in medium alone or in medium containing the indicated concentrations of various stimuli. Proliferative responses are expressed as mean counts of triplicates from a single [³H]thymidine incorporation assay; error bars indicate standard deviations. Results are representative of those from four to six experiments.