FIG. 7.

Induction of Ca²⁺ mobilization and tyrosine phosphorylation in p110δ-deficient mice. (A) Induction of Ca²⁺ was measured by flow cytometry following stimulation of Indo-1-labeled splenic B cells with anti-IgM or of splenic T cells with anti-CD3 followed by anti-IgG. Arrow 1, time point of specific stimulation; arrow 2, time point of ionomycin addition. The results are representative of those from four independent experiments. WT, wild type. (B) Purified splenic B cells from wild-type and p110δ^−/− mice were stimulated with anti-IgM (20 μg/ml) for 3 min and analyzed for the induction of tyrosine phosphorylation (pTyr) of Btk and PLCγ2. IP, immunoprecipitation.