FIG. 1.

The conserved C termini of MondoA and Mlx are required for cytoplasmic localization. (A) Domain diagrams of human MondoA and human Mlx. MCR, Mondo conserved region; TAD, transcriptional activation domain; bHLHZip, basic helix-loop-helix leucine zipper domain; DCD, dimerization and cytoplasmic localization domain. (B and C) The indicated FLAGMlx (B) and MondoA (C) constructs were expressed in NIH 3T3 cells and their subcellular localization was quantified. Nuc, predominantly nuclear; Nuc = Cyto, nuclear equivalent to cytoplasmic; Cyto, predominantly cytoplasmic.