FIG.6.
MCRII is a CRM1-dependent NES. (A) MCRII export signals from Mondo family members aligned with known export signals from the indicated proteins. Black circles denote the conserved hydrophobic residues believed to be required for NES function. The NES in MCRII fits a φ-x3-φ-x2-φ-x-φ hydrophobic residue spacing consensus, where φ is a hydrophobic amino acid and x is any amino acid. Point mutations m1 and m2 are indicated. (B) Gal alone, GalMondoA(124-143), or one of the GalMondoA(124-143) mutants was expressed in NIH 3T3 cells and the subcellular localization of the individual proteins was quantified. Nuc, predominantly nuclear; Nuc = Cyto, nuclear equivalent to cytoplasmic; Cyto, predominantly cytoplasmic. (C) Photomicrographs showing representative NIH 3T3 cells expressing GalMondoA(124-143) either without (−) or following (+) LMB treatment. (D) Gal and GalMondoA(124-143) were expressed in NIH 3T3 cells, and the subcellular localization of the individual proteins was quantified either without (−) or following (+) LMB treatment.