FIG. 7.

MCRIII of MondoA interacts with 14-3-3. (A) MondoA or MondoAm5 including a V5 epitope tag were transiently expressed in 293T cells. Anti-V5 immunoprecipitations were performed, followed by anti-V5 and anti-14-3-3 Western blotting. (B) A yeast two-hybrid assay was used to test for interaction between VP16-14-3-3β and the indicated MondoA fusions to LexA. All LexA fusions failed to interact with VP16 alone and VP16-14-3-3β also failed to interact with LexA alone (data not shown). β-Galactosidase activity was scored as follows: −, none; +, strong. (C) Gal, GalMondoA(125-321), and GalMondoA(125-321)m5 were expressed in NIH 3T3 cells, and the subcellular localization of the individual proteins was quantified without (−) or following (+) LMB treatment. Nuc, predominantly nuclear; Nuc = Cyto, nuclear equivalent to cytoplasmic; Cyto, predominantly cytoplasmic.