Table 1.
Curing and complementation by Ure2C-GFP fusion proteins
| Plasmid | URE2 part* | GFP signal | Curing,† USA−/Total | Complementation of ure2Δ |
|---|---|---|---|---|
| pH328 | 1–2, 66–354–GFP | + | 40/40 | + |
| pH409 | 1, 86–354–GFP | + | 40/40 | + |
| pH410 | 1, 106–354–GFP | + | 40/40 | − |
| pH550 | 1, 111–354–GFP | + | 40/100 | − |
| pH495 | 1, 116–354–GFP | +/− | 0/100 | − |
| pH411 | 1, 126–354–GFP | − | 0/40 | − |
| pH445 | 1–2, 66–293–GFP | + | 0/40 | − |
| pH444 | 1–2, 66–313–GFP | + | 0/40 | − |
| pH443 | 1–2, 66–333–GFP | + | 0/40 | − |
| pH494 | 1–2, 66–344–GFP | − | 0/100 | − |
| pH760 | 1–2, 66–347–GFP | + | 98/100 | − |
| pH549 | 1–2, 66–349–GFP | + | 100/100 | − |
| pH199 | –GFP | + | − |
*
The portions of Ure2p are shown as residue numbers. After the Ure2p portion is the sequence GGR followed by GFP.
†
Curing was assayed in strain YHE64 (MATα ura2 leu2 trp1 [URE3]) as USA− transformants/total. USA complementation was assayed in strain YHE887 (MATα ura2 leu2 ure2).